|
KMID : 1145120160140020201
|
|
Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 2 p.201 ~ p.211
|
|
|
Effects of Amentoflavone on Anti-inflammation and Cytoprotection
|
|
Lee Song-Jeong
Han Hyo-Sun
An In-Sook
Ahn Kyu-Joong
|
|
|
Abstract
|
|
|
Purpose: The purpose of this study was to verify the potential of amentoflavone as a material of cosmetic and medicine in oxidative stress conditions by confirming the effects of anti-inflammation and cytoprotection.
Methods: To investigate the anti-inflammatory effects of amentoflavone, the expression of inflammatory cytokines such as TNF-α and IL-6 was measured by qRT-PCR. Additionally, we performed NF-κB promoter luciferase assay to check the expression of NF-κB. To confirm the cytoprotective effects, p53 siRNA transfection was conducted to determine the relation between p53 and 14-3-3σ. Moreover, we performed cell cycle analysis to examine the effect of amentoflavone on 14-3-3σ mRNA expression and cell cycle arrest.
Results: The expression of inflammatory factors such as TNF-α, IL-6, and NF-κB was decreased in a dose dependent manner. In case of cytoprotection, amentoflavone reduced the expression of p53 mRNA. We confirmed the positive correlation between p53 and 14-3-3σ by p53 siRNA transfection. As the expression of 14-3-3σ mRNA was decreased by amentoflavone, the cell cycle progression was restored from the cell cycle arrest.
Conclusion: As mentioned above, amentoflavone decreased the expression of inflammatory and cell-damaging factors, and restored the cell cycle progression. Consequently, we found out that amentoflavone can be a valuable material possessing effects of anti-inflammation and cytoprotection in cosmetic and medicine.
|
|
|
KEYWORD
|
|
|
Amentoflavone, Anti-inflammation, Cytoprotection, Oxidative stress, Human dermal fibroblast
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|